CLASSICAL LITERATURE SEMINAR #16

 

DATE: 11 December 2006

 

TOPIC: Histology and Physiology of the Pulpodentinal Complex

 

CHAIRPERSON: LT HAWLEY

 

SEMINAR OBJECTIVE: Each resident should understand the histology and physiology of the dentin and dental pulp in order to understand the functions and reactions of the pulpodentinal complex during homeostasis and pathosis.

 

MAIN POINTS: At the conclusion of the seminar, each resident should:

 

MP #1-Describe the embryologic origins of teeth and the different stages of tooth development.

 

MP #2-Know the cellular and intercellular elements of the dental pulp.

 

MP #3-Relate the histologic appearance of each element of the dental pulp.

 

MP #4-Describe the physical and physiologic functions of each element of the dental pulp.

 

MP #5-Know the interdependence of cellular and intercellular elements of the dental pulp.

 

 

GENERAL READINGS:All residents should review and be prepared to discuss the following text and literature references.

 

Byers MR, Narhi MVO. Nerve supply of the pulpodentin complex and responses to injury. In: Hargreaves KM, Goodis HE, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:151-79.

 

D’Souza R. Development of the pulpodentin complex. In: Hargreaves KM, Goodis HE, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:13-40.

 

Eisenmann DR. Amelogenesis. In: Ten Cate R, ed. Oral histology – development, structure, and function. 5th ed. St Louis: Mosby-Year Book, Inc., 1998:197-217.

 

Fouad AF. Molecular mediators of pulpal inflammation. In: Hargreaves KM, Goodis HE, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:247-80.

 

Goodis HE. History of pulp biology. In: Hargreaves KM, Goodis HE, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:1-11.

 

Hargreaves KM. Pain mechanisms of the pulpodentin complex. In: Hargreaves KM, Goodis HE, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:181-203.

 

Kim S, Trowbridge H, Suda H. Pulpal reaction to caries and dental procedures. In: Cohen S, Burns RC, eds. Pathways of the pulp. 8th ed. St Louis: Mosby, Inc., 2002:573-602.

 

Okiji T. Pulp as a connective tissue. In: Hargreaves KM, Goodis HE, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:95-122.

 

Pashley DH, Walton RE, Slavkin HC. Histology and physiology of the dental pulp. In: Ingle JI, Bakland LK, eds. Endodontics. 5th ed. Hamilton, Ont: BC Decker Inc, 2002:25-61.

 

Suda H, Ikeda H. The circulation of the pulp. In: Hargreaves KM, Goodis, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:123-50.

 

Ten Cate AR. Dentinogenisis. In: Ten Cate AR, ed. Oral histology – development, structure, and function. 5th ed. St Louis: Mosby-Year Book, Inc., 1998:128-49.

 

Ten Cate AR. Development of the tooth and its supporting structures. In: Ten Cate AR, ed. Oral histology – development, structure, and function. 5th ed. St Louis: Mosby-Year Book, Inc., 1998:78-103.

Torneck CD. Dentin-pulp complex. In: Ten Cate AR, ed. Oral histology – development, structure, and function. 5th ed. St Louis: Mosby-Year Book, Inc., 1998;150-96.

 

Torneck CD, Torabinejad M. Biology of the dental pulp and periradicular tissues. In: Walton RE, Torabinejad M, eds. Principles and practice of endodontics. 3rd ed. Philadelphia: W.B. Saunders Company, 2002:3-22.

 

Pashley DH, Liewehr FR. Structure and functions of the dentin and pulp complex. In: Cohen S, Hargreaves KM, eds. Pathways of the pulp. 9th ed. St Louis: Mosby, Inc., 2006:460.

 

Trowbridge HO. Histology of pulpal inflammation. In: Hargreaves KM, Goodis HE, eds. Seltzer and Bender’s dental pulp. 4th ed. Chicago: Quintessence Publishing Co, Inc, 2002:227-45.

 

Gharai S. Microvasculature of the pulp. Special Report. Naval Postgraduate Dental School, Bethesda, MD. June 2002.

 

Hofmann BH.  The non-collagenous dentin matrix proteins and the biomineralization of dentin.  Special Report. Naval Postgraduate Dental School, Bethesda, MD.  January 2003.

 

Strimer, A. Antigen presentation. Special Report, Naval Postgraduate Dental School, Bethesda, MD.  October 1999.

 

 

Aubin JE. New immunological approach to studying the odontoblast. J Dent Res 1985;64 (Spec. Issue):515-22.

 

Bartold PM, Schlagenhauf U. Localization of chondroitin sulphate and dermatan sulphate in human dental pulp - an immunohistochemical study. Int Endod J 1995;28:19-24.

 

Berggreen E, Heyerass KJ. The role of sensory neuropeptides and nitric oxide on pulpal blood flow and tissue pressure in the ferret. J Dent Res 1999;78:1535-43.

 

Bernick S. Lymphatic vessels of the human dental pulp. J Dent Res 1977;56:70-7.

 

Beveridge EE, Brown AC. The measurement of human dental intra-pulpal pressure and its response to clinical variables. Oral Surg Oral Med Oral Pathol 1965;19:655-68.

 

Bishop MA, Malhotra M. An investigation of lymphatic vessels in the feline dental pulp. Am J Anat 1990;187:247-53. 

 

Butler WT, Ritchie H. The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol 1995;39:169-79.

Byers MR, Newhaus SJ, Gehrig JD. Dental sensory receptor structure in human teeth. Pain 1982;13:221-35.

 

Cutright DE, Bhaskar SN. Pulpal vasculature as demonstrated by a new method. Oral Surg Oral Med Oral Pathol 1969;27:678-83.

 

Esposito P, Varvara G, Caputi S, Perinetti G. Catalase activity in human healthy and inflamed dental pulps. Int Endod J 2003;36:599-603.

 

Fristad I, Heyeraas KJ, Kvinnsland I. Nerve fibres and cells immunoreactive to neurochemical markers in developing rat molars and supporting tissues. Arch Oral Biol 1994;39:633-46.

 

Goodis HE, Winthrop V, White JM. Pulpal responses to cooling tooth temperatures. J Endod 2000;26:263-7.

 

Hengartner MO. The biochemistry of apoptosis. Nature 2000;407:770-6.

 

Heyeraas KJ. Pulpal hemodynamics and interstitial fluid pressure: balance of transmicrovascular fluid transport. J Endod 1989;15:468-72.

 

Holt KG, Eleazer PD, Scheetz, JP. Wet and dry deep cavity preparations compared by a novel odontoblast culture technique. J Endod 2001;27:103-6.

 

Itoh K. The distribution of nerves in human deciduous and permanent teeth. Arch Histol Jpn 1976;39:379-99.

 

Johnsen DC. Innervation of teeth: qualitative, quantitative, and developmental assessment. J Dent Res 1985;64(Special Issue):555-63.

 

Kitamura C, Terashita M. Expressions of c-jun and jun-B proto-oncogenes in

odontoblasts during development of bovine tooth germs. J Dent Res 1997;76:822-30.

 

Kramer IRH. The vascular architecture of the human dental pulp. Arch Oral Biol 1969;

2:177-89.

 

Mjor IA, Svenn OB, Heyeraas KJ. Pulp-dentin biology in restorative dentistry. Part I: normal structure and physiology. Quintessence Int 2001;32:427-46.

 

Moss-Salentijn L, Hendricks-Klyvert M. Calcified structures in human dental pulps. J Endod 1988;14:184-9.

 

Murray P, About I, Lumley P, Franquin J, Remusat M, Smith A. Human odontoblast cell numbers after dental injury. J Dent 2000;28:277-85.

 

Okamura K, Kobayashi I, Matsuo K, Taniguchi K, Ishibashi Y, Izumi T, Sakai H. Ultrastructure of the neuromuscular junction of vasomotor nerves in the microvasculature of human dental pulp. Arch Oral Biol 1994;39:171-6.

 

Pohto P, Antila R. Innervation of blood vessels in the dental pulp. Int Dent J 1972;22:228-39.

 

Ruch JV. Odontoblast differentiation and the formation of the odontoblast layer. J Dent Res 1985;64(Special Issue):489-98.

 

Sloan AJ, Rutherford RB, Smith AJ. Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro. Arch Oral Biol 2000;45:173-7.

 

Spoto G, Fioroni M, Rubini C, Tripodi D, Di Stillo M, Piattelli A. Alkaline phosphatase activity in normal and inflamed dental pulps. J Endod 2001;27:180-2.

 

Thesleff I. Genetic basis of tooth development and dental defects. Acta Odontol Scand 2000;58:191-4.

 

Thomas HF. The extent of the odontoblast process in human dentin. J Dent Res 1979;58:2207-18.

 

Thomas HF. The lamina limitans of human dentinal tubules. J Dent Res 1984;63:1064-66.

 

Tziafas D. Mechanisms controlling secondary initiation of dentinogenesis: a review. Int Endod J 1994;27:61-74.

 

Ueki S, Iwai-Liao Y, Han K, Higashi Y. Histological study of the circulatory system of human dental pulp from individuals under local anesthesia and electro-acupuncture. Okajimas Folia Anat Jpn. 1995;71:335-43.

 

Van Hassel HJ. Physiology of the human dental pulp. Oral Surg Oral Med Oral Pathol 1971;32:126-35.

 

Wakisaka S. Neuropeptides in the dental pulp: distribution, origins, and correlation. J Endod 1990;16:67-9.

 

 

SEMINAR ASSIGNMENTS: All residents should review and prepare abstracts for the assigned articles and be prepared to discuss the following literature references.

 

 

LCDR SAITO

 

Fitzgerald M, Chiego DJ, Heys DR. Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth. Arch Oral Biol 1990;35:707-15.

 

Holland GR. The odontoblast process: form and function. J Dent Res 1985;64(Special Issue):499-514.

 

Ranly DM, Thomas HF, Chen J, MacDougall M. Osteocalcin expression in young and aged dental pulps as determined by RT-PCR. J Endod 1997;23:374-7.

 

Thesleff I, Vaahtokari A. The role of growth factors in determination and differentiation of the odontoblast cell lineage. Proc Finn Dent Soc 1992;88:357-68.

 

 

LCDR BLANSCET

 

Kim S, Dorscher-Kim J. Hemodynamic regulation of the dental pulp in a low compliance environment. J Endod 1989;15:404-8.

 

Murray PE, Stanley HR, Matthews JB, Sloan AJ, Smith AJ. Age-related odontometric changes of human teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:474-82.

 

Ohazama A, Modino SAC, Miletich I, Sharpe PT. Stem-cell-based tissue engineering of murine teeth. J Dent Res 2004;83:518-22.

 

Thomas HF. The dentin-predentin complex and its permeability; anatomical overview. J Dent Res 1985;64(Special Issue):607-12.

 

 

LT JACK

 

Kitasako Y, Shibata S, Cox CF, Tagami J. Location, arrangement and possible function of interodontoblastic collagen fibres in association with calcium hydroxide-induced hard tissue bridges. Int Endod J 2002;35:996-1004.

 

Koling A. Structural relationships in the human odontoblast layer as demonstrated by freeze-fracture electron microscopy. J Endod 1988;14:239-46.

 

Linde A. The extracellular matrix of the dental pulp and dentin. J Dent Res 1985;64(Special Issue):523-9.

 

Turner DF. Immediate physiological response of odontoblasts. Proc Finn Dent Soc 1992;88:55-63.

 

 

LT JOHN

 

Mangkornkarn C, Steiner JC. In vivo and in vitro glycosamino-glycans from human dental pulp. J Endod 1992;18:327-31.

 

Sigal MJ, Pitaru S, Aubin JE, Ten Cate AR. A combined scanning electron microscopy and immunofluorescence study demonstrating that the odontoblast process extends to the dentinoenamel junction in human teeth. Anat Rec 1984;210:453-62.

 

Tonder KJH, Kvinnsland I. Micropuncture measurements of interstitial fluid pressure in normal and inflamed dental pulp in cats. J Endod 1983;9:105-9.

 

Yoshiba N, Yoshiba K, Nakamura H, Iwaku M, Ozawa H. A Confocal laser scanning microscopic study of the immunofluorescent  localization of fibronectin in the odontoblast layer of human teeth. Arch Oral Biol 1994;39:395-9.

 

 

LT HORAN

 

Graham L, Cooper PR, Cassidy N, Nor JE, Sloan AJ, Smith AJ. The effect of calcium hydroxide on solubilisation of bio-active dentine matrix components. Biomaterials 2006;27:2865-73.

 

Inoue H, Kurosaka Y, Abe K. Autonomic nerve endings in the odontoblast/predentin border and predentin of the canine teeth of dogs. J Endod 1992;18:149-51.

 

Narhi M, Jyvasjarvi E, Virtanen A, Huopaniemi T, Ngassapa D, Hiroven T. Role of intradental A- and C-type nerve fibers in dental pain mechanisms. Proc Finn Dent Soc 1992;88:507-16.

 

Pashley DH. Mechanistic analysis of fluid distribution across the pulpodentin complex. J Endod 1992;18:72-5.

 

 

LCDR GUISE

 

Farnoush A. Mast cells in human dental pulp. J Endod 1984;10:250-2.

 

Kardos TB, Hunter AR, Hanlin SM, Kirk EE. Odontoblast differentiation: a response to environmental calcium? Endod Dent Traumatol 1998;14:105-11.

 

Ohazama A, Courtney JM, Sharpe PT. Opg Rank and Rankl in tooth development: co-ordinationof odontogenesis and osteogenesis. J Dent Res 2004;83:241-4.

 

Takahashi K, Kishi Y, Kim S. A scanning electron microscope study of the blood vessels of dog pulp using corrosion resin casts. J Endod 1982;8:131-5.

 

 

LCDR GNAU

 

Inoue H, Muneyuki H, Izumi T, Taguchi K, Nishigawa Y, Watanabe K, Ohkawa Y, Tamura N. Electron microscopic study on nerve terminals during dentin bridge formation after pulpotomy in dog teeth. J Endod 1997;23:569-71.

 

Kim S. Neurovascular interactions in the dental pulp in health & inflammation. J Endod 1990;16:48-53.

 

Olgart LM, Edwall B, Gazelius B. Neurogenic mediators in control of pulpal blood flow. J Endod 1989;15:409-12.

 

Trantor IR, Messer HH, Birner R. The effects of neuropeptides (calcitonin gene-related peptide and substance P) on cultured human pulp cells. J Dent Res 1995;74:1066-71.

 

 

LT CHESLER

 

Carrigan PJ, Morse DR, Furst ML, Sinai IH. A scanning electron microscopic evaluation of human dentinal tubules according to age and location. J Endod 1984;10:359-63.

 

Kim S. Regulation of pulpal blood flow. J Dent Res 1985;64(Special Issue):590-6.

 

Nair PNR. Neural elements in dental pulp and dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995;80:710-9.

 

Stanley HR, White CL, McCray L. The rate of tertiary (reparative) dentin formation in the human tooth. Oral Surg Oral Med Oral Pathol 1966;21:108-89.

 

 

 

ADDITIONAL READINGS:These references have appeared in past General Readings or as abstracted articles.  They are archived in this seminar for historical purposes and to enhance General Readings.  Although they will not be reviewed during the seminar, all residents are encouraged to read these papers.

 

Bartold PM, Moule AJ, Li H, Rigby P. Isolation and characterization of the proteoglycans synthesized by adult human pulp fibroblasts in vitro. Int Endod J 1995;28:163-71.

 

Ibutki T, Kido MA, Kiyoshima T, Tereda Y, Tanaka T. An ultrastructural study of the relationship between sensory trigeminal nerves and odontoblasts in rat dentin/pulp as demonstrated by the anterogrtade transport of WGA-HARP. J Dent Res 1996;75:1963-70.

 

Shuttleworth CA, Berry L, Kielty CM. Microfibrillar components in dental pulp: presence of both type VI collagen and fibrillin-containing microfibrils. Arch Oral Biol 1992;37:1079-84.

 

Weinstock M, Leblond CP. Synthesis, migration and release of precursor collagen by odontoblasts as visualized by radioautography after 3H-proline administration. J Cell Biol 1974;60:92.